Identification of mycobacteria species by molecular methods
نویسندگان
چکیده
منابع مشابه
Strategies for differentiation, identification and typing of medically important species of mycobacteria by molecular methods
Molecular biology methods offer new opportunities to differentiate, identify and type bacterial species and strains. These methods use the variability of nucleic sequences of genes such as 16S rDNA, beta subunit RNA-ase (rpoB), gyrase (gyrB), rDNA internal transcribed spacer and other genes. The aim of this paper is to provide comprehensive information about the methods available to differentia...
متن کاملIdentification of Nontuberculous Mycobacteria Species Isolated from Water Samples Using Phenotypic and Molecular Methods and Determination of their Antibiotic Resistance Patterns by E- Test Method, in Isfahan, Iran
Introduction Many studies have shown epidemiological links between strains isolated in tap water, and those isolated from patients. Molecular methods linked to PCR are more reliable and faster for identification of non- tuberculous mycobacteria(NTM). In this study molecular methods were used for identification and typing of NTM. Materials and Methods Five hundred ml of 85 water ...
متن کاملMolecular Identification of Rare Clinical Mycobacteria by Application of 16S-23S Spacer Region Sequencing
Objective(s) In addition to several molecular methods and in particular 16S rDNA analysis, the application of a more discriminatory genetic marker, i.e., 16S-23S internal transcribed spacer gene sequence has had a great impact on identification and classification of mycobacteria. In the current study we aimed to apply this sequencing power to conclusive identification of some Iranian clinical ...
متن کاملidentification of nontuberculous mycobacteria species isolated from water samples using phenotypic and molecular methods and determination of their antibiotic resistance patterns by e- test method, in isfahan, iran
introduction many studies have shown epidemiological links between strains isolated in tap water, and those isolated from patients. molecular methods linked to pcr are more reliable and faster for identification of non- tuberculous mycobacteria(ntm). in this study molecular methods were used for identification and typing of ntm. materials and methods five hundred ml of 85 water samp...
متن کاملIdentification of Mycobacteria species in cutaneous lesions of Sarcoidosis by PCR-Restriction Ragment Length Polymorphism (PCR-RFLP) method
Background: Sarcoidosis is a granulomatous multisystem disease of unknown etiology. It has recently been tired to detect Mycobacteria genome in biopsy specimens of patients with sarcoidosis by Polymorphism chain reaction method. Objective: To detect and identify Mycobacteria species in cutaneous lesions of the patients with sarcoidosis by PCR-RFLP. Patients and methods: 20 patients with clinica...
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ژورنال
عنوان ژورنال: International Wound Journal
سال: 2019
ISSN: 1742-4801,1742-481X
DOI: 10.1111/iwj.13238